Phospho-CGAS-S420 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-CGAS-S420 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP1228
• Supplier Catalog Number: AP1228
• Alternative Catalog Number: ABB-AP1228-1000UL, ABB-AP1228-20UL, ABB-AP1228-500UL, ABB-AP1228-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Mouse
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: Mb21d1, E330016A19Rik, Phospho-CGAS-S420

Product Description

The protein encoded by this gene is a DNA binding cytosolic protein that catalyzes the synthesis of cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) after sensing the presence of DNA in the cytoplasm. cGAMP binds another protein, Stimulator of interferon genes (STING), leading to the induction of interferons, and a host immune response. Reduced expression of this gene inhibits interferon induction in the presence of some viral infections. Alternative splicing results in multiple transcript variants.

Product Properties

• Molecular Weight: 58kDa
• NCBI: 214763
• UniProt: Q8C6L5
• Purity: Affinity purification
• Target: Cgas

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Rat

Images

Western blot analysis of various lysates using Phospho-CGAS-S420 Rabbit pAb (AP1228) at 1:1000 dilution. 293T and C6 cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.