Phospho-ATR-S428 Rabbit mAb, Unconjugated

Product Overview

• Article Name: Phospho-ATR-S428 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-AP1358
• Supplier Catalog Number: AP1358
• Alternative Catalog Number: ABB-AP1358-100UL,ABB-AP1358-20UL,ABB-AP1358-1000UL,ABB-AP1358-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: FRP1, MEC1, SCKL, FCTCS, SCKL1, Phospho-ATR-S428

Product Description

The protein encoded by this gene is a serine/threonine kinase and DNA damage sensor, activating cell cycle checkpoint signaling upon DNA stress. The encoded protein can phosphorylate and activate several proteins involved in the inhibition of DNA replication and mitosis, and can promote DNA repair, recombination, and apoptosis. This protein is also important for fragile site stability and centrosome duplication. Defects in this gene are a cause of Seckel syndrome 1.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ARC55118]
• Molecular Weight: 301 kDa
• NCBI: 545
• UniProt: Q13535
• Purity: Affinity purification
• Sequence: GISPK
• Target: ATR
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Epigenetic writers and erasers of core Histones,DNA Damage Repair,Protein phosphorylation,Cancer,Tumor suppressors,Signal Transduction,Kinase,Serine threonine kinases,Cell Biology Developmental Biology,Apoptosis,Mitochondrial Control of Apoptosis,Cell Cycle,Cell Cycle Control-G1 S Checkpoint,Cell Cycle Control-G2 M DNA Damage Checkpoint.

Images

Western blot analysis of lysates from 293T cells using Phospho-ATR-S428 Rabbit mAb (AP1358) at1:2000 dilution. 293T cells were treated with UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90 s.