Thymic stromal lymphopoietin (TSLP) is an interleukin-7 (IL-7) like cytokine, which plays an important role in the regulation of immune responses to allergens. TSLP binds to a heterodimeric receptor complex composed of the IL-7 receptor alpha chain (IL-7Ralpha) and the TSLP receptor (TSLPR, also known as CRLF2).
95 % as determined by Tris-Bis PAGE, 95 % as determined by HPLC.
Target:
TSLPR
Application Notes:
Cross-Reactivity: Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage,it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA,5% HSA,10% FBS or 5% Trehalose),and aliquot the reconstituted protein solution to minimize free-thaw cycles., ResearchArea: Other Recombinant Protein
Recombinant Human TSLPR Protein was determined by Tris-Bis PAGE under reducing conditions.
The purity of Human TSLPR is greater than 95% as determined by SEC-HPLC.
Immobilized Human TSLP (R127A, R130A) at 1µg/ml (100µl/Well). Dose response curve for Human TSLPR, hFc Tag with the EC<,sub>,50<,/sub>, of 15.5ng/ml determined by ELISA.
Immobilized Human TSLPR, hFc Tag at 0.5µg/ml (100µl/well) on the plate. Dose response curve for Biotinylated Anti-TSLPR Antibody, hFc Tag with the EC<,sub>,50<,/sub>, of 21.4ng/ml determined by ELISA.
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