Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS46) (Trehalose free), Unconjugated, Human
Biozol Catalog Number:
ABS-NUN-S46-100UG
Supplier Catalog Number:
NUN-S46-100ug
Alternative Catalog Number:
ABS-NUN-S46-100UG
Manufacturer:
AcroBiosystems
Host:
Human
Category:
Antikörper
Species Reactivity:
Mouse
Conjugation:
Unconjugated
AntiSARSCoV2 Nucleocapsid Antibody, Mouse IgG1 (AS46) (NUNS46) was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant SARSCoV2 Nucleocapsid protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. As verified by binding test with NNTD (Cat.No. NUNC5143) and NCTD (Cat.No. NUNC5145) protein, this antibody can only bind to NNTD (AA Gly 44 Glu 174). - Antikörper
Tag:
Mouse IgG1 | Mouse Kappa
Buffer:
PBS, pH7.4
Purity:
0.95
Form:
Liquid
Target:
Nucleocapsid protein
Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C5227) at 2 µg/mL (100 µL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (Cat. No. NUN-S46) with a linear range of 0.15-2.5 ng/mL (QC tested).
Detection SARS-CoV-2 Nucleocapsid Protein by Sandwich ELISA Assay.Immobilized Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS46) (Cat. No. NUN-S46) at 4 µg/mL (100 µL/well) can bind SARS-CoV-2 Nucleocapsid Protein (Cat. No. NUN-C5227). And then add Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (Cat. No. NUN-S47L8) at 0.05 µg/mL. Detection was performed using high sensitivity HRP-conjugated streptavidin with sensitivity of 12.5 pg/mL (Routinely tested).
Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS46) on SDS-PAGE under reducing (R) and under non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 0.95.
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