ONECUT1 Antibody - middle region

Product Overview

• Article Name: ONECUT1 Antibody - middle region
• Biozol Catalog Number: ASB-ARP38481_P050
• Supplier Catalog Number: ARP38481_P050
• Alternative Catalog Number: ASB-ARP38481_P050-25UL,ASB-ARP38481_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ONECUT1
• Alternative Names: HNF6, HNF-6, HNF6A

Product Description

ONECUT1 belongs to the CUT homeobox family. It contains 1 CUT DNA-binding domain and 1 homeobox DNA-binding domain. It is a transcriptional activator. ONECUT1 binds the consensus sequence 5-DHWATTGAYTWWD-3 on a variety of gene promoters such as those of HNF3B and TTR. It is important for liver genes transcription.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 51kDa
• NCBI: 3175
• UniProt: Q9UBC0
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.

Immunohistochemistry with Human Brain, cerebellum tissue at an antibody concentration of 5.0ug/ml using anti-ONECUT1 antibody (ARP38481_P050)

WB Suggested Anti-ONECUT1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Jurkat cell lysate

Host: Rabbit
Target: ONECUT1
Positive control (+): HepG2 (HG)
Negative control (-): HeLa (HL)
Antibody concentration: 3ug/ml

ONECUT1 antibody - middle region (ARP38481_P050) in Human Jurkat using Western Blot