CheKine(TM) Micro Phosphoenolpyruvate Carboxykinase (PEPCK) Activity Assay Kit provides a simple, convenient and rapid method for PEPCK activity detection, which is suitable for testing animal and plant tissues, cells, bacteria, serum (plasma) and other samples
Tag:
PEPCK
Application Notes:
CheKine(TM) Micro Phosphoenolpyruvate Carboxykinase (PEPCK) Activity Assay Kit provides a simple, convenient and rapid method for PEPCK activity detection, which is suitable for testing animal and plant tissues, cells, bacteria, serum (plasma) and other samples. The principle is that PEPCK catalyzes the oxidation of oxaloacetate to produce phosphoenolpyruvate and CO2, pyruvate kinase and lactate dehydrogenase further catalyze the oxidation of NADH to produce NAD+ in turn, The rate of NADH decline was measured at 340 nm, which reflects PEPCK activity.
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