Anti-ATP5B
Catalog Number:
ATA-HPA001528
- Images (14)
| Article Name: | Anti-ATP5B |
| Biozol Catalog Number: | ATA-HPA001528 |
| Supplier Catalog Number: | HPA001528 |
| Alternative Catalog Number: | ATA-HPA001528-100,ATA-HPA001528-25 |
| Manufacturer: | Atlas Antibodies |
| Host: | Rabbit |
| Category: | Sonstiges |
| Application: | ICC, IHC, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence |
| Conjugation: | Unconjugated |
| Alternative Names: | ATPSB |
| ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide |
| Anti-ATP5B |
| Clonality: | Polyclonal |
| Concentration: | 0.1 mg/ml |
| Isotype: | IgG |
| NCBI: | 506 |
| UniProt: | P06576 |
| Buffer: | 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. |
| Purity: | Affinity purified using the PrEST antigen as affinity ligand |
| Sequence: | LTGLTVAEYFRDQEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGTMQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRAIAELGIYPAVDPLDSTSRIMDPNIVGSEHYDVAR |
| Storage: | Store at +4°C for short term storage. Long time storage is recommended at -20°C. |
| Target: | ATP5B |
| Antibody Type: | Monoclonal Antibody |
| Application Dilute: | ICC-IF: 0.25-2 µg/ml, IHC: 1:200 - 1:500, WB: 0.04-0.4 µg/ml |
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Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria. |
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Immunohistochemical staining of human kidney, liver, prostate and small intestine using Anti-ATP5B antibody HPA001528 (A) shows similar protein distribution across tissues to independent antibody HPA001520 (B). |
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Immunohistochemical staining of human prostate shows strong granular cytoplasmic positivity in glandular cells. |
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Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules. |
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Immunohistochemical staining of human liver shows strong granular cytoplasmic positivity in hepatocytes. |
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Immunohistochemical staining of human small intestine shows moderate granular cytoplasmic positivity in glandular cells. |
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Western blot analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATP5B antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB. |
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Western blot analysis using Anti-ATP5B antibody HPA001528 (A) shows similar pattern to independent antibody HPA001520 (B). |
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Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II. |
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HPA001528-100ul |
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HPA001528-100ul |
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HPA001528-100ul |
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