Anti-SPARC

Catalog Number: ATA-HPA002989
Article Name: Anti-SPARC
Biozol Catalog Number: ATA-HPA002989
Supplier Catalog Number: HPA002989
Alternative Catalog Number: ATA-HPA002989-100,ATA-HPA002989-25
Manufacturer: Atlas Antibodies
Host: Rabbit
Category: Antikörper
Application: IHC, WB
Species Reactivity: Human
Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Conjugation: Unconjugated
Alternative Names: ON
secreted protein, acidic, cysteine-rich (osteonectin)
Anti-SPARC
Clonality: Polyclonal
Concentration: 0.1 mg/ml
Isotype: IgG
NCBI: 6678
UniProt: P09486
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Purity: Affinity purified using the PrEST antigen as affinity ligand
Sequence: VLVTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQKDIDKDL
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Target: SPARC
Antibody Type: Monoclonal Antibody
Application Dilute: IHC: 1:200 - 1:500, WB: 0.04-0.4 µg/ml
Immunohistochemical staining of human placenta shows strong positivity in endothelial cells and fibroblasts.
Western blot analysis in human cell lines U2OS and HeLa using Anti-SPARC antibody. Corresponding SPARC RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Western blot analysis using Anti-SPARC antibody HPA002989 (A) shows similar pattern to independent antibody HPA003020 (B).
HPA002989-100ul
HPA002989-100ul
HPA002989-100ul