Anti-COX5B Antibody , Unconjugated, Rabbit, Polyclonal

Catalog Number: ATA-HPA034517
Article Name: Anti-COX5B Antibody , Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: ATA-HPA034517
Supplier Catalog Number: HPA034517
Alternative Catalog Number: ATA-HPA034517-100,ATA-HPA034517-25
Manufacturer: Atlas Antibodies
Host: Rabbit
Category: Antikörper
Application: ICC, IHC, WB
Species Reactivity: Human
Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Conjugation: Unconjugated
Alternative Names: COX5B
cytochrome c oxidase subunit Vb
Anti-COX5B
Clonality: Polyclonal
Concentration: 0.5 mg/ml
Isotype: IgG
NCBI: 1329
UniProt: P10606
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Purity: Affinity purified using the PrEST antigen as affinity ligand
Sequence: ASGGGVPTDEEQATGLEREIMLAAKKGLDPYNVLAPKGASGTREDPNLVPSISNKRIVGCICEEDNTSVVWFWLHKGEAQRCPRCGAHYKLVP
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Target: COX5B
Antibody Type: Monoclonal Antibody
Application Dilute: ICC-IF: 0.25-2 µg/ml, IHC: 1:500 - 1:1000, WB: 0.04-0.4 µg/ml
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Immunohistochemical staining of human stomach shows distinct granular cytoplasmic positivity in glandular cells.
Western blot analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-COX5B antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-COX5B antibody. Remaining relative intensity is presented
Western blot analysis in human cell line HEK 293.
HPA034517
HPA034517
HPA034517