Anti-CD3E Antibody , Unconjugated, Rabbit, Polyclonal

Catalog Number: ATA-HPA043955
Article Name: Anti-CD3E Antibody , Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: ATA-HPA043955
Supplier Catalog Number: HPA043955
Alternative Catalog Number: ATA-HPA043955-100,ATA-HPA043955-25
Manufacturer: Atlas Antibodies
Host: Rabbit
Category: Antikörper
Application: IHC, WB
Species Reactivity: Human
Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Conjugation: Unconjugated
Alternative Names: Pan-Cancer
CD3e molecule, epsilon (CD3-TCR complex)
Anti-CD3E
Clonality: Polyclonal
Concentration: 0.3 mg/ml
Isotype: IgG
NCBI: 916
UniProt: P07766
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Purity: Affinity purified using the PrEST antigen as affinity ligand
Sequence: WSKNRKAKAKPVTRGAGAGGRQRGQNKERPPPVPNPDYEPIRKGQRDLYSGLNQRRI
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Target: CD3E
Antibody Type: Monoclonal Antibody
Application Dilute: IHC: 1:500 - 1:1000, WB: 0.04-0.4 µg/ml
Immunohistochemistry analysis in human lymph node and pancreas tissues using HPA043955 antibody. Corresponding CD3E RNA-seq data are presented for the same tissues.
Immunohistochemical staining of human cerebral cortex, lymph node, spleen and tonsil using Anti-CD3E antibody HPA043955 (A) shows similar protein distribution across tissues to independent antibody HPA040957 (B).
Immunohistochemical staining of human small intestine shows moderate to strong cytoplasmic positivity in lymphoid cells.
Immunohistochemical staining of human granular layer of the cerebellum shows no cytoplasmic positivity as expected.
Immunohistochemical staining of human spleen shows moderate to strong cytoplasmic positivity in cells in red pulp.
Immunohistochemical staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.
Immunohistochemical staining of human lymph node shows moderate to strong cytoplasmic positivity in lymphoid cells.
Immunohistochemical staining of human cerebral cortex using Anti-CD3E antibody HPA043955.
Immunohistochemical staining of human tonsil using Anti-CD3E antibody HPA043955.
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251 MG
Lane 4: Human plasma
Lane 5: Human Liver tissue
Lane 6: Human Tonsil tissue
HPA043955
HPA043955
HPA043955