3'-(O-Propargyl)-GTP can be incorporated in RNA at the 3end using poly(A) Polymerase. But for adding an alkyne to the 3'-end 3'-(O-Propargyl)-ATP (BCT-31) is superior. We developed a different approach in which 3'-(O-Propargyl)-GTP is added to mRNA to the 5end with an capping enzyme and afterwards methylated. As a result a cap-like structure is created which provides all the advantages of a cap structure together with the possibility to perform click reactions at the incorporated alkyne.
Molecular Weight:
561.23 g/mol (free acid)
Purity:
95% (HPLC)
Form:
Liquid
CAS Number:
[1610778-63-6]
Formula:
C13H18N5O14P3 (free acid)
Application Notes:
Triphosphate for modifying of 3END RNA
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