Melanoma Marker (MART-1 + gp100)(DT101+ BC199 + HMB45), 0.2mg/mL, Clone: [DT101 BC199 HMB45], Mouse, Monoclonal

Product Overview

• Article Name: Melanoma Marker (MART-1 + gp100)(DT101+ BC199 + HMB45), 0.2mg/mL, Clone: [DT101 BC199 HMB45], Mouse, Monoclonal
• Biozol Catalog Number: BOT-BNUB0703-500
• Supplier Catalog Number: BNUB0703-500
• Alternative Catalog Number: BOT-BNUB0703-500-500UL
• Manufacturer: Biotium
• Host: Mouse
• Category: Antikörper
• Application: IHC
• Species Reactivity: Human
• Immunogen: Recombinant hMART-1 protein (DT101 & BC199), Extract of pigmented melanoma metastases from lymph nodes (HMB45)
• Alternative Names: Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A, PMEL17

Product Description

This antibody cocktail recognizes two melanoma-specific proteins, which include MART-1 and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. A cocktail of these two markers is sensitive for labeling formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Product Properties

• Clonality: Monoclonal
• Concentration: 0.2 mg/mL
• Clone Designation: [DT101 BC199 HMB45]
• Molecular Weight: 20-22 kDa (doublet) (MART-1), 90-100 kDa (gp100)
• UniProt: Q16655
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Source: Animal

Application Notes

• Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistochemistry (formalin-fixed): 0.5-1.0 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user