TP53 Antibody, IgG1, Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: TP53 Antibody, IgG1, Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: BYT-ORB1336382
• Supplier Catalog Number: orb1336382
• Alternative Catalog Number: BYT-ORB1336382-100
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: FC, IF, IHC, WB
• Species Reactivity: Human
• Immunogen: Full length human recombinant protein of human TP53 (NP_000537) produced in HEK293T cell.
• Conjugation: Unconjugated
• Alternative Names: BCC7, LFS1, P53, TRP53

Product Description

TP53 Antibody

Product Properties

• Clonality: Monoclonal
• Clone Designation: [OTI3D5]
• Molecular Weight: 43.5 kDa
• Isotype: IgG1
• NCBI: 000546
• UniProt: P04637
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Target: TP53

Application Notes

• Application Dilute: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Application Notes: Application Notes: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process

Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TP53 (RC200003, Right lane) cDNA for 48 hrs and lysed.

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TP53 monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min)

Anti-TP53 mouse monoclonal antibody (TA502780) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TP53)

HEK293T cells transfected with either RC200003 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TP53 antibody (TA502780), and then analyzed by flow cytometry.

Flow cytometric Analysis of Hela cells, using anti-TP53 antibody (TA502780), (Red), compared to a nonspecific negative control antibody, (Blue).

Flow cytometric Analysis of Jurkat cells, using anti-TP53 antibody (TA502780), (Red), compared to a nonspecific negative control antibody, (Blue).