VGLUT1 Antibody (Biotin), IgG1, Clone: [N28/9 Formerly sold as S28-9], Mouse, Monoclonal

Article Name:	VGLUT1 Antibody (Biotin), IgG1, Clone: [N28/9 Formerly sold as S28-9], Mouse, Monoclonal
Biozol Catalog Number:	BYT-ORB149725
Supplier Catalog Number:	orb149725
Alternative Catalog Number:	BYT-ORB149725-100
Manufacturer:	Biorbyt
Host:	Mouse
Category:	Antikörper
Application:	IHC, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1
Conjugation:	Biotin
Alternative Names:	VGLUT1, Vesicular Glutamate Transporter 1, SLC17A7, BNPI, Solute Carrier Family 17 Member 7, Brain-specific Na(+)-dependent Inorganic Phosphate Cotransporter, Solute Carrier Family 17 (Sodium-Dependent Inorganic Phosphate Cotransporter), Member 7, Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7, VGluT1, VGLUT 1

Mouse monoclonal to VGlut1 (Biotin). VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate..

Clonality:	Monoclonal
Concentration:	1 mg/ml
Clone Designation:	[N28/9 Formerly sold as S28-9]
Molecular Weight:	52kDa
Isotype:	IgG1
NCBI:	446311
UniProt:	Q62634
Buffer:	136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
Target:	VGLUT1

Application Dilute:	WB (1:1000)
Application Notes:	Application Notes: 1 µg/ml of SMC-394 was sufficient for detection of VGLut1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody

	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone N28/9. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain, Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VGLUT1 Antibody (D) Composite.
	Immunohistochemistry analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone N28/9. Tissue: spinal cord. Species: Mouse. Fixation: 4% PFA. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:500 for 16 hours at RT. Secondary Antibody: Alexa Fluor 555 Donkey Anti-Mouse (red) at 1:2000 for 2 hours at RT. Counterstain: NeuN neuronal stain (green). Magnification: 20X.
	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone N28/9. Tissue: Differentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:100. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) VGLUT1 Antibody. (D) Hoechst (E) Composite.
	Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT1 protein using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone N28/9. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:1000.