Rabbit anti-P-Selectin/CD62P Recombinant Monoclonal Antibody, Clone: [BLR267L], Unconjugated

Product Overview

• Article Name: Rabbit anti-P-Selectin/CD62P Recombinant Monoclonal Antibody, Clone: [BLR267L], Unconjugated
• Biozol Catalog Number: BYT-ORB1784576
• Supplier Catalog Number: orb1784576
• Alternative Catalog Number: BYT-ORB1784576-100
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IF, IHC, WB
• Species Reactivity: Human
• Immunogen: Residues 42-771 (ECD)
• Conjugation: Unconjugated
• Alternative Names: LECAM3, leukocyte-endothelial cell adhesion molecule 3, PADGEM, platelet activation dependent granule-external membrane protein, platelet alpha-granule membrane protein, P-selectin, GRMP, PSEL, granule membrane protein 140kDa, granule membrane protein 140, GMP-140, GMP140, CD62P, CD62 antigen-like family member P, CD antigen CD62P, CD62, granulocyte membrane protein, selectin P (granule membrane protein 140kDa, antigen CD62)

Product Description

Rabbit anti-P-Selectin/CD62P Recombinant Monoclonal Antibody

Product Properties

• Clonality: Recombinant
• Concentration: 1000 µg/ml
• Clone Designation: [BLR267L]
• UniProt: P16109
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Purity: Recombinant antibody was purified from cell culture supernatant
• Form: Whole IgG
• Target: P-Selectin/CD62P

Application Notes

• Application Dilute: Immunocytochemistry (ICC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. Immunohistochemistry (IHC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Application Notes: Application Notes: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight

Images

Detection of human P-Selectin/CD62P by immunocytochemistry. Sample: FFPE section of HEL 92.1.7 cells. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784576). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human P-Selectin/CD62P by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784576). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human P-Selectin/CD62P (red) by immunofluorescence. Sample: FFPE section of metastatic lymph node. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784576) used at 1:100. Secondary: Invitrogen Goat anti-rabbit IgG Alexa Fluor(TM) Plus 647 1:400. Counterstain: DAPI (blue).

Detection of human P-Selectin/CD62P by western blot. Samples: Whole cell lysate (25 µg) from HEK293T, Platelets, Jurkat, HEL 92.1.7, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784576) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.