GABA A Receptor Antibody (Biotin), IgG2a, Clone: [N95/35 Formerly sold as S95-35], Mouse, Monoclonal

Product Overview

• Article Name: GABA A Receptor Antibody (Biotin), IgG2a, Clone: [N95/35 Formerly sold as S95-35], Mouse, Monoclonal
• Biozol Catalog Number: BYT-ORB182077
• Supplier Catalog Number: orb182077
• Alternative Catalog Number: BYT-ORB182077-100
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: AM, ICC, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Fusion protein amino acids 355-394 of human GABA-A-R-Alpha1
• Conjugation: Biotin
• Alternative Names: ECA4, EJM, EJM5, GABA receptor alpha 1 polypeptide, GABA(A) receptor, GABA(A) receptor subunit alpha 1, GABA(A) receptor subunit alpha-1, GABA(A) receptor, alpha 1, GABRA 1, GABRA1, Gamma aminobutyric acid (GABA) A receptor alpha 1, Gamma aminobutyric acid A receptor alpha 1, Gamma aminobutyric acid receptor subunit alpha 1, Gamma-aminobutyric acid receptor subunit alpha-1, GBRA1_HUMAN

Product Description

Mouse monoclonal antibody against GABA-A Receptor Alpha-1, conjugated to Biotin.

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/ml
• Clone Designation: [N95/35 Formerly sold as S95-35]
• Molecular Weight: 55kDa
• Isotype: IgG2a
• NCBI: 034380
• UniProt: P14867
• Buffer: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
• Target: GABA A Receptor

Application Notes

• Application Dilute: WB (1:1000), IHC (1:1000), ICC/IF (1:100)
• Application Notes: Application Notes: 1 µg/ml of SMC-346 was sufficient for detection of alpha1 GABA receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody

Images

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha-1 Monoclonal Antibody, Clone N95/35. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha-1 Monoclonal Antibody at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain, Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Alpha-1 Antibody (D) Composite.

Immunohistochemistry analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone N95/35. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dermal Cells.

Western Blot analysis of Human Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone N95/35. Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.