Histone H3 Monoclonal Antibody, IgG2b, Clone: [1G2E4], Unconjugated, Mouse

Catalog Number: CSB-MA010418A0M
Article Name: Histone H3 Monoclonal Antibody, IgG2b, Clone: [1G2E4], Unconjugated, Mouse
Biozol Catalog Number: CSB-MA010418A0M
Supplier Catalog Number: CSB-MA010418A0m
Alternative Catalog Number: CSB-MA010418A0M-100UL, CSB-MA010418A0M-50UL
Manufacturer: Cusabio
Host: Mouse
Category: Antikörper
Application: ELISA, IHC, IP, WB
Species Reactivity: Human, Mouse, Rabbit, Rat
Conjugation: Unconjugated
Alternative Names: Histone H3
Clonality: Monoclonal
Clone Designation: [1G2E4]
Isotype: IgG2b
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purity: >95%, Protein A purified
Form: Liquid
Target: Histone H3
Application Dilute: Recommended dilution: WB:1:5000-1:640000, IHC: 1:100-1:200, IP:2µl-5µl
Western Blot
Positive WB detected in: 10ug Hela whole cell lysate, HistoneH3 antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000, 1:1280000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 15-25 KDa
Observed band size: 15-25 KDa
Exposure time:5s
Western Blot
Positive WB detected in: Raw264.7 whole cell lysate, Raji whole cell lysate, MCF-7 whole cell lysate
All lanes: HistoneH3 antibody at 1:20000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 15-25 KDa
Observed band size: 15-25 KDa
Exposure time: 1min
Western Blot
Positive WB detected in: Rabbit stomach tissue
All lanes: HistoneH3 antibody at 1:20000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 15-25 KDa
Observed band size: 15-25 KDa
Exposure time: 1min
Western Blot
Positive WB detected in: Rabbit heart tissue, Rabbit liver tissue,Rabbit spleen tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit small intestine tissue
All lanes: HistoneH3 antibody at 1:20000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 15-25 KDa
Observed band size: 15-25 KDa
Exposure time: 2min
Western Blot
Positive WB detected in: MCF-7 whole cell lysate, Mouse heart tissue, Mouse liver tissue, Mouse lung tissue, Mouse brain tissue tissue
All lanes: HistoneH3 antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 15-25 KDa
Observed band size: 15-25 KDa
Exposure time: 5s
CSB-MA010418A0m
Western Blot
Positive WB detected in: Hela whole cell lysate at 10ug, 5ug, 2.5ug, 1.25ug, 0.625ug
All lanes: HistoneH3 antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 15-25 KDa
Observed band size: 15-25 KDa
Exposure time: 5s