KCNQ3 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA012091LA01HU
Article Name: KCNQ3 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA012091LA01HU
Supplier Catalog Number: CSB-PA012091LA01HU
Alternative Catalog Number: CSB-PA012091LA01HU-100UG, CSB-PA012091LA01HU-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, WB
Species Reactivity: Human, Mouse, Rat
Conjugation: Unconjugated
Alternative Names: BFNC 2 antibody, BFNC antibody, BFNC2 antibody, EBN 2 antibody, EBN2 antibody, KCNQ 3 antibody, KCNQ3 antibody, KCNQ3_HUMAN antibody, KQT like 3 antibody, KQT-like 3 antibody, KV7.3 antibody, Potassium channel subunit alpha KvLQT3 antibody, Potassium channel voltage gated subfamily Q member 3 antibody, Potassium voltage gated channel KQT like protein 3 antibody, Potassium voltage gated channel KQT like subfamily member 3 antibody, Potassium voltage gated channel subfamily KQT member 3 antibody, Potassium voltage-gated channel subfamily KQT member 3 antibody, Voltage gated potassium channel subunit Kv7.3 antibody, Voltage-gated potassium channel subunit Kv7.3 antibody
Clonality: Polyclonal
UniProt: O43525
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purity: Antigen Affinity Purified
Form: Liquid
Target: KCNQ3
Application Dilute: Recommended dilution: WB:1:500-1:2000,IF:1:10-1:50
Immunofluorescence staining of HepG2 cell with CSB-PA012091LA01HU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of SH-SY5Y cell with CSB-PA012091LA01HU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of SH-SY5Y cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot
Positive WB detected in: K562 whole cell lysate(30µg), THP-1 whole cell lysate(30µg), JK whole cell lysate(30µg), HepG2 whole cell lysate(30µg), A549 whole cell lysate(30µg), Mouse Brain tissue lysate(30µg),Rat Brain tissue lysate(30µg)
All lanes: KCNQ3 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 97,85,90,100 kDa
Observed band size: 100 kDa
Exposure time:120s