SPOCK1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: SPOCK1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA730785LA01HU
• Supplier Catalog Number: CSB-PA730785LA01HU
• Alternative Catalog Number: CSB-PA730785LA01HU-100UG, CSB-PA730785LA01HU-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Conjugation: Unconjugated
• Alternative Names: SPOCK1 antibody, SPOCK antibody, TIC1 antibody, TICN1 antibody, Testican-1 antibody, Protein SPOCK antibody

Product Properties

• Clonality: Polyclonal
• UniProt: Q08629
• Buffer: Preservative: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
• Purity: Antigen Affinity Purified
• Form: Liquid
• Target: SPOCK1

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200

Images

IHC image of CSB-PA730785LA01HU diluted at 1:100 and staining in paraffin-embedded human endometriall cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

Western Blot
Positive WB detected in: 293T whole cell lysate (20µg), U87 whole cell lysate (20µg), U251 whole cell lysate (20µg), SY5Y whole cell lysate (20µg), PC-3 whole cell lysate (20µg), Mouse brain tissue lysate (20µg), Rat brain tissue lysate (20µg)
All lanes: SPOCK1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa

IHC image of CSB-PA730785LA01HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

IHC image of CSB-PA730785LA01HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

IHC image of CSB-PA730785LA01HU diluted at 1:100 and staining in paraffin-embedded human Cervical cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody