RMND1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA882123LA01HU
Article Name: RMND1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA882123LA01HU
Supplier Catalog Number: CSB-PA882123LA01HU
Alternative Catalog Number: CSB-PA882123LA01HU-100UG, CSB-PA882123LA01HU-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IHC, WB
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: bA351K16 antibody, bA351K16.3 antibody, C6orf96 antibody, chromosome 6 open reading frame 96 antibody, FLJ20627 antibody, MGC117362 antibody, MGC149570 antibody, MGC882602 antibody, required for meiotic nuclear division 1 homolog (S. cerevisiae) antibody, Required for meiotic nuclear division protein 1 homolog antibody, Rmnd1 antibody, RMND1_HUMAN antibody
Clonality: Polyclonal
UniProt: Q9NWS8
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: RMND1
Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200
IHC image of CSB-PA882123LA01HU diluted at 1:50 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
IHC image of CSB-PA882123LA01HU diluted at 1:50 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
IHC image of CSB-PA882123LA01HU diluted at 1:50 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
Western Blot
Positive WB detected in: MCF7 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), THP-1 whole cell lysate(30µg)
All lanes: RMND1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 52,28,24 kDa
Observed band size: 52 kDa
Exposure time: 120s