ATF2 Recombinant Monoclonal Antibody, Clone: [3D12], Unconjugated, Rabbit

Product Overview

• Article Name: ATF2 Recombinant Monoclonal Antibody, Clone: [3D12], Unconjugated, Rabbit
• Biozol Catalog Number: CSB-RA002270A0HU
• Supplier Catalog Number: CSB-RA002270A0HU
• Alternative Catalog Number: CSB-RA002270A0HU-100UL, CSB-RA002270A0HU-50UL
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IHC, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: Cyclic AMP-dependent transcription factor ATF-2, Activating transcription factor 2, Cyclic AMP-responsive element-binding protein 2, CREB-2, cAMP-responsive element-binding protein 2, HB16, Histone acetyltransferase ATF2, cAMP response element-binding protein CRE-BP1, ATF2, CREB2, CREBP1

Product Properties

• Clonality: Monoclonal
• Clone Designation: [3D12]
• UniProt: P15336
• Buffer: Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
• Purity: Affinity-chromatography
• Form: Liquid
• Target: ATF2
• Antibody Type: Recombinant Antibody

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200

Images

IHC image of CSB-RA002270A0HU diluted at 1:115.5 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot
Positive WB detected in: K562 whole cell lysate, 293T whole cell lysate
All lanes: ATF2 antibody at 1.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 55, 36, 24, 49, 53, 14, 25, 16 KDa
Observed band size: 70 KDa