ENO1 Recombinant Monoclonal Antibody, Clone: [8H9G12], Unconjugated

Product Overview

• Article Name: ENO1 Recombinant Monoclonal Antibody, Clone: [8H9G12], Unconjugated
• Biozol Catalog Number: CSB-RA007670MA1HU
• Supplier Catalog Number: CSB-RA007670MA1HU
• Alternative Catalog Number: CSB-RA007670MA1HU-100UL, CSB-RA007670MA1HU-50UL
• Manufacturer: Cusabio
• Category: Antikörper
• Application: ELISA, FC, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Conjugation: Unconjugated
• Alternative Names: 2 phospho D glycerate hydro lyase antibody, 2-phospho-D-glycerate hydro-lyase antibody, Alpha enolase antibody, Alpha enolase like 1 antibody, Alpha-enolase antibody, C myc promoter binding protein antibody, C-myc promoter-binding protein antibody, EC 4.2.1.11 antibody, eno1 antibody, ENO1L1 antibody, ENOA_HUMAN antibody, Enolase 1 (alpha) antibody, Enolase 1 (alpha) like 1 antibody, Enolase 1 antibody, Enolase alpha antibody, MBP 1 antibody, MBP-1 antibody, MBP1 antibody, MBPB1 antibody, MPB 1 antibody, MPB-1 antibody, MPB1 antibody, MYC promoter binding protein 1 antibody, NNE antibody, Non neural enolase antibody, Non-neural enolase antibody, Phosphopyruvate hydratase antibody, Plasminogen binding protein antibody, Plasminogen-binding protein antibody, PPH antibody, Tau crystallin antibody

Product Properties

• Clonality: Monoclonal
• Clone Designation: [8H9G12]
• UniProt: P06733
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: Affinity-chromatography
• Form: Liquid
• Target: ENO1
• Antibody Type: Recombinant Antibody

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:2000, IHC:1:50-1:200, IF:1:50-1:200, FC:1:50-1:200

Images

Overlay Peak curve showing Hela cells stained with CSB-RA007670MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcgamma fragment specific at 1:200 dilution for 35min at 4°C.Control antibody (green line) was human IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

Immunofluorescence staining of HepG2 cell with CSB-RA007670MA1HU at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcgamma fragment specific.

Immunofluorescence staining of MCF-7 cell with CSB-RA007670MA1HU at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcgamma fragment specific.

IHC image of CSB-RA007670MA1HU diluted at 1:50 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.

Western Blot
Positive WB detected in: HeLa whole cell lysate(20µg) , U-251MG whole cell lysate(20µg) , A549 whole cell lysate(20µg) , PC-3 whole cell lysate(20µg) , K562 whole cell lysate(20µg) , NIH/3T3 whole cell lysate(20µg) , HEK293 whole cell lysate(20µg) , Jurkat whole cell lysate(20µg) , HepG2 whole cell lysate(20µg)
All lanes: ENO1 antibody at 1:1000
Secondary
Goat polyclonal to human IgG at 1/40000 dilution
Predicted band size: 47 kDa
Observed band size: 55 kDa
Exposure time:5s

Western Blot
Positive WB detected in: Mouse brain tissue lysate(20µg) , Rat brain tissue lysate(20µg) , Mouse kidney tissue lysate(20µg) , Rat kidney tissue lysate(20µg) , Mouse lung tissue lysate(20µg) , Mouse spleen tissue lysate(20µg)
All lanes: ENO1 antibody at 1:1000
Secondary
Goat polyclonal to human IgG at 1/40000 dilution
Predicted band size: 47.2 kDa
Observed band size: 55 kDa
Exposure time: 2min