NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Western Blot analysis of Jurkat, K562 cells using Acetyl-NFkappaB-p65 (K218) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody(catalog:RS0002) was diluted at 1:20000
Western Blot analysis of MOUSE-BRAIN cells using Acetyl-NFkappaB-p65 (K218) Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog:RS0002) was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded human-breast, antibody was diluted at 1:100
The picture was kindly provided by our customer,antibody was diluted at 1:500
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