HIF-1-alpha (9U11) Rabbit Monoclonal Antibody, Unconjugated
Catalog Number:
EKL-AMRE12020
Product Overview
Article Name:
HIF-1-alpha (9U11) Rabbit Monoclonal Antibody, Unconjugated
Biozol Catalog Number:
EKL-AMRE12020
Supplier Catalog Number:
AMRe12020
Alternative Catalog Number:
EKL-AMRE12020-50UL, EKL-AMRE12020-100UL, EKL-AMRE12020-200UL
Manufacturer:
EnkiLife
Host:
Rabbit
Category:
Antikörper
Application:
ICC, IP, WB
Species Reactivity:
Human, Mouse, Rat
Conjugation:
Unconjugated
Alternative Names:
HIF1, MOP1, PASD8, bHLHe78, HIF-1alpha, HIF1-ALPHA, HIF1A
Product Description
Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5-[AG]CGTG-3 within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Functions as a master transcriptional regulator of the adaptive response to hypoxia (PubMed:11292861, PubMed: 11566883, PubMed: 15465032, PubMed: 16973622, PubMed: 17610843, PubMed: 18658046, PubMed: 20624928, PubMed: 22009797, PubMed: 9887100, PubMed: 30125331). Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia (PubMed: 11292861, PubMed: 11566883, PubMed: 15465032, PubMed: 16973622, PubMed: 17610843, PubMed: 20624928, PubMed: 22009797, PubMed: 9887100, PubMed: 30125331). Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease (PubMed: 22009797). Heterodimerizes with ARNT, heterodimer binds to core DNA sequence 5-TACGTG-3 within the hypoxia response element (HRE) of target gene promoters (By similarity). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300 (PubMed: 9887100, PubMed: 16543236). Activity is enhanced by interaction with NCOA1 and/or NCOA2 (PubMed: 10594042). Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP (PubMed: 10202154, PubMed:
Product Properties
Clonality:
Monoclonal
Molecular Weight:
93kDa
NCBI:
3091
UniProt:
Q16665
Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4C short term. Store at -20C long term. Avoid freeze / thaw cycle.
Purity:
Affinity purification
Form:
Liquid
Target:
HIF1A
Application Notes
Application Dilute:
WB 1:500-1:2000,ICC/IF 1:200-1:500,IP 1:20-1:50
