PI 3 kinase p110 alpha (8X14) Rabbit Monoclonal Antibody, Unconjugated

Product Overview

• Article Name: PI 3 kinase p110 alpha (8X14) Rabbit Monoclonal Antibody, Unconjugated
• Biozol Catalog Number: EKL-AMRE16092
• Supplier Catalog Number: AMRe16092
• Alternative Catalog Number: EKL-AMRE16092-50UL, EKL-AMRE16092-100UL, EKL-AMRE16092-200UL
• Manufacturer: EnkiLife
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Conjugation: Unconjugated
• Alternative Names: PI 3 Kinase catalytic subunit alpha, phosphoinositide-3-kinase catalytic alpha polypeptide, PI3-kinase p110 alpha, PI3K, PI3K p110-alpha, PK3CA, PIK3CA, PtdIns-3-kinase p110

Product Description

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) and its phosphorylated derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed:15135396, PubMed:23936502, PubMed:28676499). Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed:15135396, PubMed:28676499). PIP3 plays a key role by recruiting PH domain- containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. In addition to its lipid kinase activity, it displays a serine-protein kinase activity that results in the autophosphorylation of the p85alpha regulatory subunit as well as phosphorylation of other proteins such as 4EBP1, H-Ras, the IL-3 beta c receptor and possibly others (PubMed:23936502, PubMed:28676499). Plays a role in the positive regulation of phagocytosis and pinocytosis (By similarity).

Product Properties

• Clonality: Monoclonal
• Molecular Weight: 124kDa
• NCBI: 5290
• UniProt: P42336
• Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4C short term. Store at -20C long term. Avoid freeze / thaw cycle.
• Purity: Affinity purification
• Form: Liquid
• Target: PIK3CA

Application Notes

• Application Dilute: WB 1:1000-1:5000,ICC/IF 1:100-1:200,IP 1:20-1:50