RPE65 (5T1) Rabbit Monoclonal Antibody, Unconjugated
Catalog Number:
EKL-AMRE17356
Product Overview
Article Name:
RPE65 (5T1) Rabbit Monoclonal Antibody, Unconjugated
Biozol Catalog Number:
EKL-AMRE17356
Supplier Catalog Number:
AMRe17356
Alternative Catalog Number:
EKL-AMRE17356-50UL, EKL-AMRE17356-100UL, EKL-AMRE17356-200UL
Manufacturer:
EnkiLife
Host:
Rabbit
Category:
Antikörper
Application:
IF, IHC, IP, WB
Species Reactivity:
Human, Mouse, Rat
Conjugation:
Unconjugated
Alternative Names:
LCA2, mRPE65, p63, rd12, Retinal pigment epithelium specific 61 kDa protein, RP20, RPE65, sRPE65,
Product Description
Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore (PubMed:16116091). Essential for the production of 11-cis retinal for both rod and cone photoreceptors (PubMed: 17848510). Also capable of catalyzing the isomerization of lutein to meso-zeaxanthin an eye- specific carotenoid (PubMed: 28874556). The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all- trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT (By similarity).
Product Properties
Clonality:
Monoclonal
Molecular Weight:
61kDa
NCBI:
6121
UniProt:
Q16518
Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4C short term. Store at -20C long term. Avoid freeze / thaw cycle.
Purity:
Affinity purification
Form:
Liquid
Target:
RPE65
Application Notes
Application Dilute:
WB 1:500-1:2000,IHC 1:500-1:2000,IP 1:20-1:50,IF-P 1:500-1:2000
