Cell-permeable derivative of E-64c, an inhibitor of calpain and other cysteine proteases, such as papain, cathepsin B and cathepsin L.1,2 Addition to cell cultures should be done in serum-free media as esterases in serum will cleave the ethyl ester and reduce cell permeability. Typical working concentration is 0.5-10 µg/ml. Inhibits degradation of autophagic cargo inside autolysosomes3
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