ATP citrate lyase antibody [N1N2], N-term, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: ATP citrate lyase antibody [N1N2], N-term, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX112387
• Supplier Catalog Number: GTX112387
• Alternative Catalog Number: GTX112387-100,GTX112387-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human ATP citrate lyase. The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: ATP citrate lyase , ACL , ATPCL , CLATP

Product Properties

• Clonality: Polyclonal
• Concentration: 0.55 mg/ml (Please refer to the vial label for the specific concentration.)
• Molecular Weight: 121
• NCBI: 47
• UniProt: P53396
• Buffer: 1XPBS (pH7), 20% Glycerol, 0.025% ProClin 300.
• Purity: Purified by antigen-affinity chromatography.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX112387 IHC-P Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATP citrate lyase antibody [N1N2], N-term (GTX112387) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ATP citrate lyase antibody [N1N2], N-term detects ATP citrate lyase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ATP citrate lyase protein stained by ATP citrate lyase antibody [N1N2], N-term (GTX112387) diluted at 1:500.
Blue: Hoechst 33342 staining.

Immunohistochemical analysis of paraffin-embedded Hepatoma, using ATP citrate lyase(GTX112387) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATP citrate lyase antibody [N1N2], N-term (GTX112387) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.