TRAF6 antibody [N2C2], Internal, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: TRAF6 antibody [N2C2], Internal, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX113029
• Supplier Catalog Number: GTX113029
• Alternative Catalog Number: GTX113029-100,GTX113029-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IHC-P, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein encompassing a sequence within the center region of human TRAF6. The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: TNF receptor associated factor 6 , MGC:3310 , RNF85

Product Properties

• Clonality: Polyclonal
• Concentration: 3.39 mg/ml (Please refer to the vial label for the specific concentration.)
• Molecular Weight: 60
• NCBI: 7189
• UniProt: Q9Y4K3
• Buffer: 1XPBS pH7, 20% Glycerol, 0.025% ProClin 300.
• Purity: Purified by antigen-affinity chromatography.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX113029 WB Image

Sample (30 μg of whole cell lysate)
A: A549
B: HeLa
C: HepG2
7.5% SDS PAGE
GTX113029 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TRAF6 protein stained by TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:100.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

TRAF6 antibody [N2C2], Internal immunoprecipitates TRAF6 protein in IP experiments.
IP samples: K562 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of TRAF6 protein by 4 μg TRAF6 antibody [N2C2], Internal (GTX113029)
7.5 % SDS-PAGE
The immunoprecipitated TRAF6 protein was detected by TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

TRAF6 antibody [N2C2], Internal detects TRAF6 protein by western blot analysis.
A. 30 μg PC-12 whole cell extract
B. 30 μg Rat2 whole cell extract
7.5% SDS-PAGE
TRAF6 antibody [N2C2], Internal (GTX113029) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded 59T xenograft, using TRAF6(GTX113029) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm in mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: BCL-1
7.5% SDS PAGE
GTX113029 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.