Methylated cytosine DNA standard kit

Product Overview

• Article Name: Methylated cytosine DNA standard kit
• Biozol Catalog Number: GTX400004
• Supplier Catalog Number: GTX400004
• Alternative Catalog Number: GTX400004-1
• Manufacturer: GeneTex
• Category: Kits/Assays
• Application: dot
• Alternative Names: 5-methylcytosine | 5-hydroxymethylcytosine | 5-formylcytosine | 5-carboxylcytosine

Product Description

Methylated cytosine DNA standard kit

Product Properties

• Concentration: 50ng/µl
• Pubmed: , 34413196
• Buffer: 10mM Tris-HCl (pH8.0), 0.1mM EDTA

Images

GeneTex methylated cytosine DNA standard kit includes five recombinant double-strand DNA fragments: unmethylated cytosine DNA (C), 5-methylated cytosine DNA (5mC), 5-hydroxymethycytosine cytosine DNA (5hmC), 5-formylcytosine cytosine DNA (5fC), 5-carboxylcytosine cytosine DNA (5caC). Each methylated cytosine DNA was spotted onto a positively charged nylon membrane and subjected to Dot blot assay with corresponding antibodies.
The membrane was incubated with anti-5hmc monoclonal antibody (GTX629701) at a dilution of 1:500 or with anti-5mc monoclonal antibody (GTX629448) at a dilution of 1:500

All five standards are 426 bp with all cytosines either unmodified (lane 1, C), 5-methylcytosine (lane 2, 5mC), 5-hydroxymethylcytosine (lane 3, 5hmC), 5-formylcytosin (lane4, 5fC), 5-carboxycytosine (lane5, 5-caC). DNA marker: 1 kb Plus DNA marker.