p53 antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: GTX50438
Article Name: p53 antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: GTX50438
Supplier Catalog Number: GTX50438
Alternative Catalog Number: GTX50438-100
Manufacturer: GeneTex
Host: Rabbit
Category: Antikörper
Application: ICC, IHC-P, IP, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Peptide sequence around aa.13~17 (P-L-S-Q-E) derived from human p53.
Conjugation: Unconjugated
Alternative Names: tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle ar
Clonality: Polyclonal
Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Clone Designation: Polyclonal
Molecular Weight: 44
Isotype: IgG
NCBI: 7157
Pubmed: 32890980,
UniProt: P04637
Buffer: PBS (without Mg and Ca) pH7.4, 150mM NaCl, 50% Glycerol, 0.02% Sodium azide.
Source: Human
Purity: Purified by antigen-affinity chromatography.
Form: Liquid
Application Notes: WB: 1:500-1:1000. ICC/IF: 1:100-1:200. IHC-P: 1:50-1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
IP analysis of extracts from 293T cells using GTX50438 p53 antibody.
WB analysis of extracts from mouse brain tissue using GTX50438 p53 antibody.
IHC-P analysis of rat breast tissue sections using GTX50438 p53 antibody.
IHC-P analysis of rat colon tissue sections using GTX50438 p53 antibody.
IHC-P analysis of rat breast tissue using GTX50438 p53 antibody.
Dilution : 1:100
ICC/IF analysis of methanol-fixed HeLa cells using GTX50438 p53 antibody.
GTX50438 WB Image
IHC-P analysis of human breast carcinoma tissue using GTX50438 p53 antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody (GTX50438) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.