Whole-cell lysate of 293T cells overexpressing SARS-CoV spike protein (full-length, no tag). The translated amino acid sequence is based on AAP13567.1. Lysate prepared in RIPA buffer with no reducing agents and is unboiled. Please see matched control 293
Concentration:
1 mg/ml (Please refer to the vial label for the specific concentration.)
Buffer:
50 mM Tris-HCl (pH8.0), 150 mM NaCl, 0.5% sodium deoxycholate, 1% Triton X-100, 0.25% SDS, 1 mM PMSF, 5 µg/ml Leupeptin, 2 µg/ml Aprotinin, 1 µg/ml Pepstatin A, 0.025% ProClin 300.
Form:
Liquid
GTX535668 WB Image
Non-transfected and transfected 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARS-CoV Spike (SΔ3) antibody [7G12] (GTX632603) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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