WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX630396 WB Image
NDP52 antibody [GT422] detects NDP52 protein at autophagosome by immunofluorescent analysis. Samples: Hep G2 cells mock (left) and treated with 3 μM Thapsigargin for 16 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with 100% MeOH for 30 sec. Green: NDP52 protein stained by NDP52 antibody [GT422] (GTX630396) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody [GT422] (GTX630396) diluted at 1:1000.
Immunoprecipitation of NDP52 protein from Jurkat whole cell extracts using 5 μg of NDP52 antibody [GT422] (GTX630396). Western blot analysis was performed using NDP52 antibody [GT422] (GTX630396). EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX630396) diluted by 1:500. The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
NDP52 antibody [GT422] detects NDP52 protein by western blot analysis. A. 30 μg Jurkat whole cell lysate/extract B. 30 μg Raji whole cell lysate/extract C. 30 μg NCI-H929 whole cell lysate/extract 10 % SDS-PAGE NDP52 antibody [GT422] (GTX630396) dilution: 1:1000
NDP52 antibody [GT422] detects NDP52 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: NDP52 protein stained by NDP52 antibody [GT422] (GTX630396) diluted at 1:1000. Red: Phalloidin, a F-actin marker.
NDP52 antibody [GT422] detects NDP52 protein by western blot analysis. A. 30 μg Huh7 whole cell lysate/extract B. 30 μg Hep3B whole cell lysate/extract C. 30 μg HepG2 whole cell lysate/extract 10 % SDS-PAGE NDP52 antibody [GT422] (GTX630396) dilution: 1:1000
NDP52 antibody [GT422] detects NDP52 protein at autophagosome? by immunofluorescent analysis.Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NDP52 stained by NDP52 antibody [GT422] (GTX630396) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10 μm.
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