Recombinant Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody, Clone: [KRT7/8121R], Rabbit, Monoclonal

Catalog Number: NBT-3855-RBM17-P0
Article Name: Recombinant Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody, Clone: [KRT7/8121R], Rabbit, Monoclonal
Biozol Catalog Number: NBT-3855-RBM17-P0
Supplier Catalog Number: 3855-RBM17-P0
Alternative Catalog Number: NBT-3855-RBM17-P0-20,NBT-3855-RBM17-P0-100
Manufacturer: NeoBiotechnologies
Host: Rabbit
Category: Antikörper
Application: IHC
Species Reactivity: Human
Immunogen: Recombinant fragment (around aa1-200) of human KRT7 protein (exact sequence is proprietary)
Alternative Names: CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7
Cytokeratin 7 in humans is a protein encoded by the KRT7 gene. It is always as a keratin pair with Cytokeratin 19. Cytokeratin 7 is expressed on most ductal and glandular epithelium including lung, breast, bladder and female genital tract, but not in most gastrointestinal epithelium, prostate, hepatocyte and squamous epithelium. In tumor tissues, its expression is absent in colon cancer, prostate cancer and squamous carcinomas. Cytokeratin 7 is often used in concert with Cytokeratin 20 and CDX-2 to aid in distinguishing ovarian, pulmonary and breast carcinomas (CK7+) from colon carcinomas (CK7-).
Clonality: Monoclonal
Clone Designation: [KRT7/8121R]
Molecular Weight: 55kDa
NCBI: 3855
UniProt: P08729
Form: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type: Recombinant Monoclonal Antibody
Application Notes: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes),Optimal dilutio
Formalin-fixed, paraffin-embedded human lung carcinoma stained with Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody (KRT7/8121R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human placenta stained with Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody (KRT7/8121R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.