MGEA5 Antibody / OGA / O-GlcNAcase, Rabbit, Polyclonal

Product Overview

• Article Name: MGEA5 Antibody / OGA / O-GlcNAcase, Rabbit, Polyclonal
• Biozol Catalog Number: NSJ-F54180-0.2ML
• Supplier Catalog Number: F54180-0.2ML
• Alternative Catalog Number: NSJ-F54180-0.2ML
• Manufacturer: NSJ Bioreagents
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Human
• Immunogen: A portion of amino acids 236-269 from human MGEA5 was used as the immunogen for the MGEA5 antibody.

Product Description

Isoform 1 (Cytoplasmic): Cleaves GlcNAc but not GalNAc from O- glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4- methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl- beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714).Isoform 3 (Nuclear): Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1. [UniProt]

Product Properties

• Clonality: Polyclonal
• Concentration: In 1X PBS, pH 7.4, with 0.09% sodium azide
• Isotype: Rabbit Ig
• UniProt: O60502
• Buffer: In 1X PBS, pH 7.4, with 0.09% sodium azide
• Purity: Antigen affinity
• Form: In 1X PBS, pH 7.4, with 0.09% sodium azide
• Target: MGEA5
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: Western blot: 1:1000-1:2000
• Application Notes: The stated application concentrations are suggested starting points. Titration of the MGEA5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

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