Acetyl-Histone H3-K9 pAb, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Acetyl-Histone H3-K9 pAb, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: PRS-22-814
• Supplier Catalog Number: 22-814
• Alternative Catalog Number: PRS-22-814-100
• Manufacturer: ProSci
• Host: Rabbit
• Category: Antikörper
• Application: ChIP, IF, IHC, IP, WB
• Species Reactivity: Mouse, Rat
• Immunogen: A synthetic peptide of human Acetyl-Histone H3-K9
• Conjugation: Unconjugated
• Alternative Names: H3F3A, H3t, H3.4, H3/g, H3FT

Product Properties

• Clonality: Polyclonal
• Concentration: batch dependent
• Molecular Weight: Observed: 16kDa
• NCBI: 8290
• UniProt: Q16695
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Form: Liquid

Application Notes

• Application Dilute: Optimal dilutions for each application to be determined by the researcher.
• Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:50

Images

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K9 antibody (22-814) at dilution of 1:100 (40x lens). NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours (left). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K9 antibody (22-814) at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37°C for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Immunohistochemistry of paraffin-embedded human mammary cancer using Acetyl-Histone H3-K9 antibody (22-814) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H3-K9 antibody (22-814) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H3-K9 antibody (22-814) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using Acetyl-Histone H3-K9 antibody (22-814) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K9 antibody (22-814) at dilution of 1:100 (40x lens). HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours (left). Blue: DAPI for nuclear staining.

Chromatin immunoprecipitation analysis of extracts of 293 cell line, using H3K9ac antibody (22-814) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.