PAX6 Antibody, IgG1, Clone: [7H10.D3.D4.G5], Mouse, Monoclonal

Product Overview

• Article Name: PAX6 Antibody, IgG1, Clone: [7H10.D3.D4.G5], Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-301-R22S
• Supplier Catalog Number: 200-301-R22S
• Alternative Catalog Number: ROC-200-301-R22S
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: ELISA, IF
• Species Reactivity: Human
• Immunogen: PAX6 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein corresponding to human PAX6 protein.
• Conjugation: Unconjugated
• Alternative Names: mouse anti-pax6 antibody, Paired box protein Pax-6, Aniridia type II protein, Oculorhombin, AN2, PAX 6 Antibody

Product Properties

• Clonality: Monoclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Clone Designation: [7H10.D3.D4.G5]
• Isotype: IgG1
• NCBI: 5080
• UniProt: P26367
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:75,000 - 1:150,000, IF Microscopy: 1:100, WB: 1:500 - 1:2,000
• Application Notes: Anti-PAX6 antibody has been tested by ELISA and Immunofluorescence and suitable for Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 46.7 kDa in size corresponding to PAX6 by western bl

Images

Immunofluorescence Microscopy of Mouse anti-PAX6 antibody. Tissue: Hela Cell,20X. Fixation: 4% PFA room temp for 10 min. Antigen retrieval: not required. Primary antibody: PAX6 antibody at 1:100 for 1 h at RT. Secondary antibody: goat anti-mouse secondary antibody at 1:1,000 for 45 min at RT. Localization: PAX6 is nuclear. Staining: PAX6 as red fluorescent signal with DAPI (blue) nuclear counterstain. Images courtesy of Yang Xiang with Boston Childrens Hospital.