Anti-DFF45 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 20 amino acid peptide near the N-terminus of human DFF45.
Conjugation:
Unconjugated
Alternative Names:
DFF45 Antibody, DFF1, ICAD, DFF-45, DFF1, DFF45, H13, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000, WB: 1:1,000
Application Notes:
Anti-DFF45 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 37 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of Rabbit anti-DFF45 antibody. Lane 1: HeLa cell lysate using anti-DFF45 antibody at 1 µg/mL (A). Lane 2: HeLa cell lysate using anti-DFF45 antibody at 2 µg/mL (B). Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 36.5 kDa, 45 kDa for DFF45. Other band(s): DFF45 splice variants and isoforms.
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