Anti-GAPDH antibody was prepared from chicken egg fractions produced by repeated immunizations with a 16 amino acid synthetic peptide from near the N-terminus of human GAPDH.
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000-1:20,000, WB: 1-2 µg/mL
Application Notes:
Anti-GAPDH Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 36 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of GAPDH antibody. Lane 1: HeLa cell lysate with GAPDH antibody at 1 µg/mL. Lane 2: HeLa cell lysate with GAPDH antibody at 2 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase chicken secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 36 kDa, 39 kDa for GAPDH. Other band(s): GAPDH splice variants and isoforms.
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