Anti-ITCH antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid peptide near the N-terminus of human ITCH.
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000 - 1:20,000, WB: 1-2 µg/mL
Application Notes:
Anti-ITCH Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 103 kDa in Western Blots of specific cell lysates and tissues.
Western blot analysis of ITCH. Load: 3T3 cell lysate. Primary Antibody: ITCH antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.
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