H1N1 Neuraminidase Antibody was produced from whole rabbit serum prepared by repeated immunizations with synthetic peptides from the seasonal influenza (H1N1) Neuraminidase protein and the novel swine influenza Neuraminidase protein. The antibody recognizes both variants.
Conjugation:
Unconjugated
Alternative Names:
Influenza A (H1N1) Neuraminidase Antibody, Influenza A (H1N1) Neuraminidase, Influenza N1, flu N1, NA
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1 ug/mL
Application Notes:
Anti-H1N1 Neuraminidase Antibody is tested for use in ELISA. Expect a band approximately ~51.6 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
ELISA of Anti-H1N1 Neuraminidase. Coating Antigen: H1N1 Neuraminidase recombinant protein at 2 µg/mL incubated at 4 C overnight. Detection Primary antibody: Anti-H1N1 Neuraminidase antibody at 1:1000 ng/mL at RT for 1 hr. Secondary Primary antibody: Goat anti-rabbit at 1:10,000, incubated at RT for 1 hr.
Western Blot of Anti-H1N1 Neuraminidase.Load: 50ng (Lane 1) and 100ng (Lane 2) H1N1 Neuraminidase recombinant protein. Primary antibody: Anti-H1N1 NA antibody at 2 µg/mL for 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
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