Rabbit IgG anti-Sheep IgG (F(ab)2)-HRPO, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Sheep IgG (F(ab)2)-HRPO, MinX none
• Biozol Catalog Number: ROC-613-4304
• Supplier Catalog Number: 613-4304
• Alternative Catalog Number: ROC-613-4304
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Sheep
• Immunogen: Sheep IgG F(ab)2 fragment
• Conjugation: HRPO
• Alternative Names: Rabbit Anti-Sheep IgG F(ab)2 peroxidase Conjugated Antibody, Rabbit Anti-Sheep IgG Fab2 peroxidase Conjugated Antibody, Rabbit Anti-Sheep IgG Fab2 Fragment Antibody HRP Conjugation
• Format: IgG
• Target Specificity: IgG (F(ab')2)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.5 mg/mL by UV absorbance at 280 nm
• Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Sheep
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Application Notes: This product has been assayed against 1.0 µg of Sheep IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1

Images

Immunohistochemistry results using rabbit anti-sheep IgG F(ab)2 HRP. Antigen retrieval was performed by heating the paraffin sections in an autoclave at 121C for 15 min. The paraffin sections were then treated with 10% normal sheep serum for staining against anti-human TSLP antibody. After several washes with PBS, the sections were stained with Peroxidase-conjugated affinity-purified anti-sheep IgG F(ab)2 secondary antibody (p/n 613-4304) for 1.5 h. The samples were treated with 0.3% H2O2 in cold methanol for 30 min. Finally, 0.1 mg/ml 3,3'-diaminobenzidine tetrahydrochloride was applied to sections for 10 min. The sections were counterstained with hematoxylin. (a, b) Immunohistochemical localization of Lag-positive cells in Hassals corpuscles of the thymus at 5 months (a, brown) and 8 months (b, dark blue) of human ontogeny. (c) Immunohistochemical localization of TSLP-R (brown, arrowheads) in Hassals corpuscles of the thymus at 5 months of gestation. Bars 10 µm. Fig. 2. PMID: 16670919.