Swine IgG Fc Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal

Product Overview

• Article Name: Swine IgG Fc Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-614-4303
• Supplier Catalog Number: 614-4303
• Alternative Catalog Number: ROC-614-4303
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA
• Species Reactivity: Porcine
• Immunogen: Swine IgG F(c) fragment
• Conjugation: HRP
• Alternative Names: Rabbit Anti-Swine IgG F(c) peroxidase Conjugated Antibody, Rabbit Anti-Swine IgG Fc peroxidase Conjugated Antibody, rabbit Anti-Swine IgG Fc Fragment Antibody HRP Conjugation

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Swine
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:171,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Swine IgG F(c) Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot co

Images

ELISA Results of Rabbit Anti-Swine IgG F(c) Antibody Peroxidase Conjugated tested against purified Swine IgG F(c) HRP. Each well was coated in duplicate with 1.0 µg of Swine IgG F(c) (p/n 014-0104) [Red Line] and Swine IgG (p/n 014-0102) [Green Line]. The working dilution of Swine IgG F(c) is 1:219,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076) and TMB substrate (p/n TMBE-1000).