Aldehyde Dehydrogenase 1-A1, Recombinant, Human, aa1-501

Product Overview

• Article Name: Aldehyde Dehydrogenase 1-A1, Recombinant, Human, aa1-501
• Biozol Catalog Number: USB-046041
• Supplier Catalog Number: 046041
• Alternative Catalog Number: USB-046041-20,USB-046041-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Aldehyde dehydrogenase 1A1 (ALDH1A1), also known as retinal dehydrogenase 1, is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. ALDH1A1 also belongs to the group of corneal crystallins that help maintain the transparency of the cornea. (Retinal + NAD+ + H2O = retinoate + NADH) Recombinant ALDH1A1 protein was expressed in E. coli and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa1-501 from human Aldehyde Dehydrogenase 1-A1, expressed in E.coli. Molecular Weight: ~54.8kD (501aa) Amino Acid Sequence: MSSSGTPDLP VLLTDLKIQY TKIFINNEWH DSVSGKKFPV FNPATEEELC QVEEGDKEDV DKAVKAARQA FQIGSPWRTM DASERGRLLY KLADLIERDR LLLATMESMN GGKLYSNAYL NDLAGCIKTL RYCAGWADKI QGRTIPIDGN FFTYTRHEPI GVCGQIIPWN FPLVMLIWKI GPALSCGNTV VVKPAEQTPL TALHVASLIK EAGFPPGVVN IVPGYGPTAG AAISSHMDID KVAFTGSTEV GKLIKEAAGK SNLKRVTLEL GGKSPCIVLA DADLDNAVEF AHHGVFYHQG QCCIAASRIF VEESIYDEFV RRSVERAKKY ILGNPLTPGV TQGPQIDKEQ YDKILDLIES GKKEGAKLEC GGGPWGNKGY FVQPTVFSNV TDEMRIAKEE IFGPVQQIMK FKSLDDVIKR ANNTFYGLSA GVFTKDIDKA ITISSALQAG TVWVNCYGVV SAQCPFGGFK MSGNGRELGE YGFHEYTEVK TVTVKISQKN S Biological Activity: Specific activity is > 700pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole propioaldehyde by NAD/ minute at pH 8.8 at 37°C. Applications: Suitable for use in SDS-PAGE, Enzyme Activity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 54.8
• NCBI: 000680
• UniProt: P00352
• Purity: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL).
• Form: Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 10% glycerol.