GRPEL1, Recombinant, Human, aa1-217, GST-Tag (GrpE Protein Homolog 1, Mitochondrial)
Biozol Catalog Number:
USB-373534
Supplier Catalog Number:
373534
Alternative Catalog Number:
USB-373534-20, USB-373534-100, USB-373534-1
Manufacturer:
US Biological
Category:
Molekularbiologie
Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins. Source: Recombinant protein corresponding to aa1-217 from human GRPEL1, fused to GST-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~48.3kD Amino Acid Sequence: CTATKQKNSGQNLEEDMGQSEQKADPPATEKTLLEEKVKLEEQLKETVEKYKRALADTENLRQRSQKLVEEAKLYGIQAFCKDLLEVADVLEKATQCVPKEEIKDDNPHLKNLYEGLVMTEVQIQKVFTKHGLLKLNPVGAKFDPYEHEALFHTPVEGKEPGTVALVSKVGYKLHGRTLRPALVGVVKEA Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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