PAT, Recombinant, Streptomyces Viridochromogenes, aa1-183, His-SUMO-Tag (Phosphinothricin N-acetyltransferase)

Product Overview

• Article Name: PAT, Recombinant, Streptomyces Viridochromogenes, aa1-183, His-SUMO-Tag (Phosphinothricin N-acetyltransferase)
• Biozol Catalog Number: USB-374625
• Supplier Catalog Number: 374625
• Alternative Catalog Number: USB-374625-20,USB-374625-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Inactivates phosphinothricin (PPT) by transfer of an acetyl group from acetyl CoA. This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance. Source: Recombinant protein corresponding to aa1-183 from streptomyces viridochromogenes PAT, fused to His-SUMO-Tag at N-terminal expressed in E. coli. Molecular Weight: ~36.6kD Amino Acid Sequence: MSPERRPVEIRPATAADMAAVCDIVNHYIETSTVNFRTEPQTPQEWIDDLERLQDRYPWLVAEVEGVVAGIAYAGPWKARNAYDWTVESTVYVSHRHQRLGLGSTLYTHLLKSMEAQGFKSVVAVIGLPNDPSVRLHEALGYTARGTLRAAGYKHGGWHDVGFWQRDFELPAPPRPVRPVTQI Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 36.6
• UniProt: Q57146
• Purity: ~90% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.