RpiA, Recombinant, E. coli, aa1-219, GST-Tag (Ribose-5-phosphate Isomerase A)

Product Overview

• Article Name: RpiA, Recombinant, E. coli, aa1-219, GST-Tag (Ribose-5-phosphate Isomerase A)
• Biozol Catalog Number: USB-375093
• Supplier Catalog Number: 375093
• Alternative Catalog Number: USB-375093-20,USB-375093-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Involved in the first step of the non-oxidative branch of the pentose phosphate pathway. It catalyzes the reversible conversion of ribose-5-phosphate to ribulose 5-phosphate. Can also act on D-ribose-5-diphosphate and D-ribose-5-triphosphate as substrate. Source: Recombinant protein corresponding to aa1-219 from E. coli rpiA, fused to GST-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~49.9kD Amino Acid Sequence: MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKGQIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTREKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGVVTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIVK Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 49.9
• UniProt: P0A7Z0
• Purity: 90% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.