Polymerase Acidic Protein, Recombinant, Influenza A virus, aa124-247, His-Tag (PA)

Product Overview

• Article Name: Polymerase Acidic Protein, Recombinant, Influenza A virus, aa124-247, His-Tag (PA)
• Biozol Catalog Number: USB-406022
• Supplier Catalog Number: 406022
• Alternative Catalog Number: USB-406022-20,USB-406022-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5 cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity. Source: Recombinant protein corresponding to aa124-247 from Influenza A virus Polymerase acidic protein, fused to His-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~18.6kD Amino Acid Sequence: RREVHIYYLEKANKIKSEKTHIHIFSFTGEEMATKADYTLDEESRARIKTRLFTIRQEMASRGLWDSFRQSERGEETIEERFEITGTMRKLADQSLPPNFSSLENFRAYVDGFEPNGYIEGKLS Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 18.6
• UniProt: Q9IQ47
• Purity: ~90% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.