MitoPT JC-1 BioAssay(TM) Kit

Product Overview

• Article Name: MitoPT JC-1 BioAssay(TM) Kit
• Biozol Catalog Number: USB-474033
• Supplier Catalog Number: 474033
• Alternative Catalog Number: USB-474033-100,USB-474033-400
• Manufacturer: US Biological
• Category: Kits/Assays
• Application: FC

Product Description

Sample Type: Cell culture Reagent Name: JC-1 Target: Mitochondrial depolarization Excitation/Emission: 488nm/590nm, 527nm Method of Analysis: Flow Cytometry, Fluorescence Microscope, Fluorescence Plate Reader Intended Use: The MitoPT JC-1 BioAssay(TM) detects mitochondrial membrane depolarization utilizing the fluorescent dye JC-1. When accumulated in a negatively charged polarized mitochondria, JC-1 fluoresces orange. When mitochondrial membrane potential collapses in apoptotic or metabolically stressed cells, JC-1 reagent is dispersed through the cell and will fluoresce green. Analyze your results using a flow cytometer, fluorescence plate reader, or fluorescence microscopy. Test Principle: Loss of mitochondrial membrane potential is indicative of apoptosis. MitoPT JC-1 BioAssay(TM) utilizes the potentiometric fluorescent dye JC-1 to detect mitochondrial membrane permeability and membrane depolarization. The JC-1 dye has a delocalized positive charge dispersed throughout its molecular structure. In addition, its lipophilic solubility enables it to be readily membrane permeant and penetrate living cells. The JC-1 dye enters the negatively charge mitochondria where it accumulates and fluoresces orange upon excitation. When the mitochondrial membrane potential collapses in apoptotic cells, MitoPT JC-1 becomes distributed throughout the cytosol. Analyze your results using a flow cytometer, fluorescence plate reader, or fluorescence microscopy. Kit Components: 100 tests: MitoPT JC-1 Reagent, 100 Tests 10X Assay Buffer, 1x60mL CCCP, 50mM, 1x125uL 400 tests: MitoPT JC-1 Reagent, 400 Tests 10X Assay Buffer, 2x125mL CCCP, 50mM, 600uL Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.