Clathrin Light Chain A, Recombinant, Mouse, aa1-235, His-Tag, Flag-Tag

Product Overview

• Article Name: Clathrin Light Chain A, Recombinant, Mouse, aa1-235, His-Tag, Flag-Tag
• Biozol Catalog Number: USB-584013
• Supplier Catalog Number: 584013
• Alternative Catalog Number: USB-584013-20,USB-584013-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. Source: Recombinant protein corresponding to aa1-235 from mouse Clathrin light chain A, fused to 6X His-Tag at N-terminal and Flag-Tag at C-terminal, expressed in Mammalian cell. Molecular Weight: ~31.1kD Amino Acid Sequence: MAELDPFGAPAGAPGGPALGNGVAGAGEEDPAAAFLAQQESEIAGIENDEAFAILDGGAPGRATRRAAGGPDAVDGVMNGEYYQESNGPTDSYAAISEVDRLQSEPESIRKWREEQTERLEALDANSRKQEAEWKEKAIKELEEWYARQDEQLQKTKANNRVADEAFYKQPFADLIGYVAAEEAFVNDIDESSPGTEWERVARLCDFNPKSSKQAKDVSRMRSVLISLKQAPLVH Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 31.1
• UniProt: O08585
• Purity: 90% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.